Daniel Zalewski

Academic Researcher
Bioinformatician
R programmer
Pharmacist
Laboratory Diagnostician

About me

Hi, I'm Daniel and I work as a researcher in the Medical University of Lublin in Poland.In my research, I focus on the identification of transcriptomic biomarkers of peripheral vascular diseases to explain their pathogenesis and propose diagnostic and therapeutic targets. I use such technologies as NGS sequencing, RT-qPCR, ELISA, and for data analysis and visualisation, I mainly use an excellent R environment.Working in innovative fields with amazing scientists allows me to make relevant discoveries, which is a great reward and a source of motivation and bravery to go further.

My research/Academic career

October 2022 - presentResearch Associate ProfessorMedical University of Lublin, Poland
March 2022 - September 2022Research Adiunct ProfessorMedical University of Lublin, Poland
March 2022 - presentPostgraduate course in Data ScienceWarsaw University of Technology
October 2021 - February 2022Research Assistant ProfessorMedical University of Lublin, Poland
February 2019 - September 2021Research/Didactic Assistant ProfessorMedical University of Lublin, Poland
October 2019 - September 2020Postgraduate course in Data AnalysisMaria Curie- Sklodowska University in Lublin, Poland
October 2017 - July 2019Postgraduate course in Medicinal Laboratory, Laboratory Diagnostician LicenseMedical University of Lublin, Poland
October 2015 - September 2019PhD in PharmacyMedical University of Lublin, Poland
March 2013 - June 2015PharmacistMelisa pharmacy, Lublin, Poland
October 2007 - January 2013MSc in PharmacyMedical University of Lublin, Poland
October 2008 - September 2012Chairman of the Research Student Group "Biotechnolog"Medical University of Lublin, Poland

My top favourite tool

I love R and R Studio programming environment for great elasticity and computative powerful. I can calculate all I want and make beutiful and tidy visualisations, dashbords and reports!

Other tools I like

Tableau

Power BI

SQL

Python

Projects I participate

2024/ABM/03/KPO (January 2025 - present)Development of the novel diagnostic test for early detection of abdominal aortic aneurysm basing on the molecular predictive biomarkers.
POWER (December 2021 - January 2022)Integrated development program of the Medical University of Lublin, 2nd edition (educational project).
Innovation Incubator 4.0 (January 2022 - October 2022)Validation of selected miRNA markers for diagnosis of human diseases using qPCR.
Miniatura 5 (November 2021 - November 2022)The analysis of miRNA expression profiles in plasma of patients with lower extremities arterial disease, abdominal aortic aneurysm and chronic venous disease using Next Generation Sequencing.
STRATEGMED 3 (July 2018 - December 2020)Development of optimized methods of treating tissue damage based on innovative composites and mesenchymal stem cells and their derivatives in patients with civilization diseases using Next Generation Sequencing.
BIOSTRATEG 3 (February 2019 - May 2019)Bioproducts from lignocellulosic biomass derived from marginal land to fill the gap in current national bioeconomy.
Other (since 2015)miRNA, transcriptomic and proteomic biomarkers associated with human peripheral vascular diseases and their risk factors. miRNA-dependent mechanisms of glaucoma. Alzheimer disease-related genes after stroke. Genetic factors of human longevity. Natural compounds with antiparasitic and anticancer activity.

I am a creator of RQdeltaCT R package – a new tool for relative quantification of gene expression using delta Ct methods. This tool:

  • balances comprehensive functionality, flexibility of programming, and ease of use,

  • offers the possibility to perform analysis using the 2-dCt and 2-ddCt methods to compare either independent groups of samples or groups with paired samples, and using both normalised or unnormalised data,

  • provides many functions for convenient data import to R, multi-step quality control of data, and diverse visualisation methods.

  • allows the users to perform additional analysis (correlation analysis, Receiver Operating Characteristic analysis, linear and logistic regression) that make the whole analysis complete,

  • was developed with special attention paid to beginners to R,

  • includes the vignette that provide step-by-step detailed instructions on how to perform the entire analysis process.

  • is publicly accessible at the GitHub (https://github.com/Donadelnal/RQdeltaCT) and CRAN (https://CRAN.R-project.org/package=RQdeltaCT) repositories.

My publications

2024

  • Key Regulators of Angiogenesis and Inflammation Are Dysregulated in Patients with Varicose Veins. International Journal of Molecular Sciences 2023, 25, 6785. Website

  • Content of selected polyphenolic compounds in four Sambucus nigra l. cultivars in relation to their antimicrobial activity. Acta Poloniae Pharmaceutica - Drug Research 2024, 6, 80. Website

  • Chemical Composition, and Antioxidant and Antimicrobial Activity of Oregano Essential Oil. Molecules 2024, 29, 435. Website

2023

  • Dysregulations of key regulators of angiogenesis and inflammation in abdominal aortic aneurysm. International Journal of Molecular Sciences 2023, 24, 12087. Website

  • The landscape of gene expression during hyperfilamentous biofilm development in oral Candida albicans isolated from a lung cancer patient. International Journal of Molecular Sciences 2023, 24, 368. Website

  • Next-generation sequencing in the assessment of the transcriptomic landscape of DNA damage repair genes in abdominal aortic aneurysm, chronic venous disease and lower extremity artery disease. International Journal of Molecular Sciences 2023, 24, 551. Website

2022

  • miRNA regulatory networks associated with peripheral vascular diseases. Journal of Clinical Medicine 2022, 11, 3470. Website

  • MicroRNA expression biomarkers of chronic venous disease. Current Issues in Pharmacy and Medical Sciences 2022, Volume 35, Issue 1. Website

  • Relationships between Indicators of Lower Extremity Artery Disease and MiRNA Expression in Peripheral Blood Mononuclear Cells. Journal of Clinical Medicine 2022, 11, 1619. Website

  • Application of OpenArray RT-QPCR for Identification of MicroRNA Expression Signatures of Lower Extremity Artery Disease. J Appl Genetics 2022. WebsitePhytochemical profiling and biological activity of the extracts obtained from green biomass of three Miscanthus L. species using supercritical carbon dioxide extraction. Industrial Crops and Products 2022, 189, 115641. Website

  • Identification of transcriptomic biomarkers of lower extremity artery disease. 8th Central European Congress of Life Sciences Eurobiotech. Kraków, 20th-22nd June 2022 Website

  • Variation in the antimicrobial activity of essential oils from cultivars of Lavandula angustifolia and L. × intermedia. Agronomy 2022, 12, 2955. Website

  • Przewlekła choroba żylna w aspekcie pracy kosmetologa. Interdyscyplinarne wyzwania nauk o zdrowiu. T. 2, pp. 607-612. Website

  • Patent nr 241808, Zastosowanie markerów mikroRNA do wykrywania obecności tętniaków aorty brzusznej oraz sposób diagnozowania tętniaków aorty brzusznej. Website

  • Patent nr 241807, Zastosowanie markerów mikroRNA do diagnozowania choroby żylnej oraz sposób diagnozowania choroby żylnej. Website

  • Patent nr 241800, Zastosowanie ekstraktów nadkrytycznych (scCO2+H2O) pozyskanych z roślin z rodzaju Miscanthus do wytwarzania preparatów o działaniu nicieniobójczym. Website

  • Patent nr 241801, Zastosowanie ekstraktów nadkrytycznych (scCO2+H2O) pozyskanych z roślin z rodzaju Miscanthus do wytwarzania preparatów o działaniu przeciwwirusowym i wirusobójczym. Website

  • Patent nr 241802, Zastosowanie ekstraktów nadkrytycznych (scCO2+H2O) pozyskanych z roślin z rodzaju Miscanthus do wytwarzania preparatów o działaniu przeciwnowotworowym. Website

  • Patent nr 241258, Zastosowanie ekstraktów roślinnych z kory i drewna topoli Populus nigra x P. maximowiczii pozyskanych metodą ekstrakcji nadkrytycznej do wytwarzania preparatu w leczeniu parazytoz ludzi i zwierząt. Website

  • Patent nr 241257, Zastosowanie ekstraktów roślinnych z kory i drewna wierzby wiciowej (Salix viminalis) pozyskanych metodą ekstrakcji nadkrytycznej do wytwarzania preparatu w leczeniu parazytoz ludzi i zwierząt. Website

2021

  • Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens. Int J Mol Sci 2021, 22. Website

  • The Correlation of Mutations and Expressions of Genes within the PI3K/Akt/MTOR Pathway in Breast Cancer - A Preliminary Study. International Journal of Molecular Sciences 2021, 22, 2061. Website

  • MicroRNAs in the Aqueous Humor of Patients with Different Types of Glaucoma. Graefe’s Archive for Clinical and Experimental Ophthalmology 2021. Website

  • Serum Peroxiredoxin-1 in Patients Undergoing Carotid Endarterectomy – Short Report. Acta Angiologica 2021, 27, 49–52. Website

  • Biomarkers of Brain Ischemia-Reperfusion Injury after Carotid Endarterectomy. Acta Angiologica 2021, 27, 113–119. Website

  • Aktywność przeciwpasożytnicza. Olejki eteryczne w profilaktyce i leczeniu chorób infekcyjnych. Red. nauk. Anna Malm, s. 72-93, Wydawnictwo Lekarskie PZWL, Warszawa, 2021. Website

2020

  • Dysregulations of MicroRNA and Gene Expression in Chronic Venous Disease. J Clin Med 2020, 9. Website

  • Dysregulation of MicroRNA Modulatory Network in Abdominal Aortic Aneurysm. J Clin Med 2020, 9. Website

  • Choroba tętnic kończyn dolnych jako jedna z przyczyn niepełnosprawności osób dorosłych. Medycyna spersonalizowana 2020. Red. Anna Bogucka-Kocka, Janusz Kocki, s. 247-256, Wydawnictwo Muzyczne POLIHYMNIA Sp. z o.o., Lublin 2020.

2019

  • Dysregulation of MicroRNA Regulatory Network in Lower Extremities Arterial Disease. Front Genet 2019, 10, 1200. Website

  • Patent: Sposób diagnozowania miażdżycy tętnic oraz zastosowanie markerów miRNA do diagnozowania miażdżycy tętnic, nr 234498 Website

  • Cytotoxic and Proapoptotic Activity of Sanguinarine, Berberine, and Extracts of Chelidonium Majus L. and Berberis Thunbergii DC. toward Hematopoietic Cancer Cell Lines. Toxins 2019, 11, 485. Website

  • Wpływ chorób pasożytniczych na funkcje poznawcze oraz zachowanie i rozwój dzieci. Wybrane zagadnienia z medycyny spersonalizowanej. Red. Piotr Goliszek, Andrzej Kiciński i Janusz Kocki, s. 109-118. Stowarzyszenie Absolwentów i Przyjaciół Wydziału Prawa Katolickiego Uniwersytetu Lubelskiego, Lublin, 2019, s. 247-256, Wydawnictwo Muzyczne POLIHYMNIA Sp. z o.o., Lublin 2020.

2017

  • Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium Majus L. Using Thin-Layer Chromatographic-Densitometric Method. Acta Chromatographica 2017, 29, 385–397. Website

Me in Polish media

  • Gold medal for our patent during XVI International Invention and Innovation Show INTARG®2023 - Link1

  • Our project won! Article1 Article2 Video

  • Our patent was awarded! Read article Read article

  • Article in academic Blask magazine about my Miniatura 5 project - Read article

  • New project within Innovation Incubator 4.0 has begun - Read article

  • Educational film about ticks and Lyme borreliosis with my small role - Watch part 1 (with me) Watch part 2

  • Gold medal and International Federation of Inventors Associations award for our patent during XIII International Invention and Innovation Show and Competition INTARG®2020 Online ‘Innovations drive the economy’ - Link1 Link2

  • My interview in Polish Radio Lublin regarding our first patent - Interview

In my free time

Table tennis

Sport shooting

Rich-story computer games

Contact

Kindly use this email address [email protected] or the form provided below.

Thank you for sending a massage to me!
I will contact you as soon as possible.
Have a nice day!